ubiquitin e4i2j rabbit mab (Cell Signaling Technology Inc)
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Ubiquitin E4i2j Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ubiquitin+antibody/pm41951141-82-10-14
Average 86 stars, based on 1 article reviews
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Western Blot:Article Title: All-Trans Retinoic Acid Modulates MicroRNA-129-5p/Hypoxia-Inducible Factor-1α/Vascular Endothelial Growth Factor Signaling and Suppresses Ets-1 in Retinal Pigment Epithelial ARPE-19 Cells. Article Snippet: Purpose: Proliferative vitreoretinopathy (PVR) is a vision-threatening complication of retinal detachment or ocular trauma characterized by the formation of contractile fibrotic membranes.. Retinal pigment epithelium (RPE) cells are central to PVR pathogenesis, driving maladaptive wound-healing responses.. This study investigated the effects of all-trans retinoic acid (ATRA) on RPE cell proliferation, vascular endothelial growth factor (VEGF) secretion, and miR-129-5p biogenesis, alongside the downstream regulation of Ets-1 and the hypoxiainducible factor-1a (HIF-1a)/VEGF axis. Article Title: The SDC1-ENO1 Axis in Cancer-Associated Fibroblasts Generates a Lactate-Rich Microenvironment that Drives Tumor Radioresistance Article Snippet: D ow naded rom http://aacrjournals.orgarticle-pdf/doi/10.1158/0008-5472.C AN -25-3806/35/can-25-3806.pdf by gest on 15 M arch 2026 Radiotherapy resistance remains a major barrier to effective treatment of triple-negative breast cancer (TNBC), highlighting the need to identify mechanisms driving resistance.. Here, we identified SDC1 as a pivotal mediator of cancer-associated fibroblast (CAF)-induced radioresistance in breast cancer.. SDC1 bound the TIM barrel domain of the glycolytic enzyme ENO1, preventing FBXW7-mediated degradation and driving aerobic glycolysis and lactate accumulation. Article Title: UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytes Article Snippet: .. To further investigate this observation, western blot analysis with an Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4. Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside. Article Title: Circ_0068481 promotes pulmonary arterial smooth muscle cell remodeling via PTEN degradation and PI3K/AKT signaling activation. Article Snippet: Objective: Pulmonary arterial hypertension (PAH) is a progressive vascular disease characterized by pulmonary vascular remodeling and elevated pulmonary artery pressure.. Although circ_0068481, a circular RNA, has been implicated in PAH, its functional roles and underlying mechanisms remain unclear.. This study aimed to investigate the role of circ_0068481 in PAH-associated cellular dysfunction and to explore its potential molecular Isolation:Article Title: UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytes Article Snippet: .. To further investigate this observation, western blot analysis with an Control:Article Title: UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytes Article Snippet: .. To further investigate this observation, western blot analysis with an In Vitro:Article Title: UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytes Article Snippet: .. To further investigate this observation, western blot analysis with an Ubiquitin Proteomics:Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4. Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside. Immunoprecipitation:Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4. Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside. Centrifugation:Article Title: Myc and Skp2 overexpression promotes p27 ubiquitination and degradation in Ewing Sarcoma Article Snippet: Cell lysates were prepared using Pierce IP Lysis Buffer (Thermo Fisher Scientific, Cat. #87788). .. After centrifugation, supernatants were incubated with anti-p27 antibody (3686, CST) at 4 °C overnight, followed by Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h. After washing, immunocomplexes were analyzed by SDS-PAGE and immunoblotted with Incubation:Article Title: Myc and Skp2 overexpression promotes p27 ubiquitination and degradation in Ewing Sarcoma Article Snippet: Cell lysates were prepared using Pierce IP Lysis Buffer (Thermo Fisher Scientific, Cat. #87788). .. After centrifugation, supernatants were incubated with anti-p27 antibody (3686, CST) at 4 °C overnight, followed by Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h. After washing, immunocomplexes were analyzed by SDS-PAGE and immunoblotted with SDS Page:Article Title: Myc and Skp2 overexpression promotes p27 ubiquitination and degradation in Ewing Sarcoma Article Snippet: Cell lysates were prepared using Pierce IP Lysis Buffer (Thermo Fisher Scientific, Cat. #87788). .. After centrifugation, supernatants were incubated with anti-p27 antibody (3686, CST) at 4 °C overnight, followed by Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h. After washing, immunocomplexes were analyzed by SDS-PAGE and immunoblotted with |

